Figure 6
Role of NO in herbicide/fungicide-induced neuronal death in mesencephalic primary cultures. A, Histological images of mesencephalic mixed cultures. Immunostaining for dopamine transporter (DAT, red), parkin (green), and Hoechst dye (blue). Scale bars: 20 μm. B, Protein levels of parkin measured by western blot of cell lysates. C, Images of primary cultures exposed to a combination of 100 μM paraquat (PQ) and 10 μM maneb (MB), with 1 mM Nω-nitro-L-arginine (NNA) or vehicle for 6 h. Staining for TUNEL (red) and Hoechst (blue). Scale bar: 10 μm. Quantification of the percentage of TUNEL-positive nuclei in each experimental condition shows PQ/MB induces cell death while NNA inhibits PQ/MB-induced death. Values are mean + SEM of 100–341 nuclei per condition performed in triplicate experiments; * p < 0.05.