Dopamine synthesis, neuronal survival, neurogenesis and astrocytosis in a mouse model of PD. A, Histological images of brain slices from mice treated with PQ/MB, the nNOS inhibitor 3-Br-7-NI, or PQ/MB with 3-Br-7-NI. Staining for TH, NeuN, MAP2, PCNA or GFAP are shown in the indicated brain regions. Scale bars: 75 μm (TH), 35 μm (NeuN), and 25 μm (MAP2, PCNA, GFAP). B, Quantification of number of cells staining for TH, NeuN and PCNA; quantitative confocal immunoreactivity for MAP2; and quantitative optical density for GFAP under the conditions indicated in the specified brain regions. Values are mean + SEM, n = 5–6 mice per condition; * p < 0.05.