Skip to main content
Figure 2 | Molecular Neurodegeneration

Figure 2

From: Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse model

Figure 2

HDAC3 and ataxin-7 physically interact and colocalize. (A) Immunoprecipitation of HEK293T cells transiently transfected with ataxin-7-10Q (wild type) or ataxin-7-92Q (expanded), or vector. Whole cell lysates were subject to immunoprecipitation with an antibody to ataxin-7 (PA1-749), separated by SDS-PAGE and immunoblotted with an antibody to HDAC3 (N-19). (B) Reverse immunoprecipation with HDAC3 (B-12) and western blotting with ataxin-7 (K) supports the binding of ataxin-7 to HDAC3. (C) Immunocytochemistry of HEK293T cells transiently transfected with ataxin-7-10Q (wild type) or ataxin-7-92Q (expanded), and co-transfected with HDAC3. Cells were fixed and stained with antibodies to ataxin-7 conjugated to Alexa 488 (green), HDAC3 conjugated to Alexa 555 (red), and counterstained with DAPI (blue). Cells were imaged using confocal microscopy to elucidate colocalization of ataxin-7 and HDAC3. Scale bar represents 10 μm.

Back to article page