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Figure 6 | Molecular Neurodegeneration

Figure 6

From: Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse model

Figure 6

HDAC3 expression in SCA7 tissue. (A) Protein was extracted from cerebellar tissue of 10.5-month-old MoPrP-flag-92Q-myc transgenic mice and non-transgenic littermates and subjected to SDS-PAGE and Western blotting with an antibody to HDAC3. GAPDH was used as a loading control. (B) Protein bands were measured by densitometry and the expression from each genotype is denoted as an average of their HDAC3 density values (n = 3) normalized to GAPDH and shown as a ratio to non-transgenic values. Bars represent standard deviation from the mean and asterisks is significance at p < 0.05. (C) Immunohistochemical staining of 9-month-old MoPrP-flag-10Q-myc and MoPrP-flag-92Q-myc transgenic mice and non-transgenic littermate controls. Fixed cerebellar tissue sections were probed with an antibody to HDAC3 conjugated to Alexa 488. Scale bar represents 50 μm.

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Molecular Neurodegeneration

ISSN: 1750-1326