Figure 7

Acetylation in SCA7 transgenic mice. (A) Immunohistochemical staining of 10.5-month-old SCA7 transgenic mice and nontransgenic littermate controls. Fixed retinal tissue sections were probed with an antibody to acetyl-lysine conjugated to Alexa 488 (green) and counterstained with DAPI (blue). Scale bar represents 20 μm. (B) HDAC activity in the retina in vivo. HDAC activity was detected in situ using a deacetylase substrate, particularly in the pigment epithelium and segment layers of the retina. Activity due to sirtuins (HDAC III class) was similar to buffer control (panels shown represent activity in SCA7-10Q tissue) as detected by HDAC I/II class specific inhibition with TSA (100 μM). Arrowheads indicate fluorescent cells. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. Scale bar represents 10 μm.