Expression of HSF1 and distribution of HSPs in spinal cord. A) Western blot of HSF1 in spinal cord homogenates from normal and symptomatic control mice normalized with HSC70, demonstrating the over-expression of HSF1 in H46R/H48QxHSF1 mice compared to H46R/H48Q littermates. B) Spinal cords were extracted with detergents of increasing ionic strength as described in the methods. S1 and P1-3 extracts were immunoblotted for inducible HSP70 (B) or αB-crystallin (C). Asterisks are given at the position of the expected monomeric protein. p is indicated by brackets, bars represent the mean of 6 mice +/- SD.