Effect of HSF1 over-expression on body weight loss and healthspan of H46R/H48Q mice. H46R/H48Q mice (Solid line) n = 20 and H46R/H48QxHSF1 (dashed line) n = 19. A) Beginning at 3 weeks of age, weekly averages of body weight are plotted for the survival cohort for WT (green circles), HSF1 (blue squares), H46R/H48Q (red triangles), and H46R/H48QxHSF1 (gold triangles). Asterisks indicate significant differences in H46R/H48QxHSF1 vs. H46R/H48Q at the *p<0.05, **p<0.01, and ***p<0.001 level. B) Disease onset was determined as the time animals reached their maximum bodyweight. C) Early disease was defined as a drop of 10% of the mouse maximal weight. D) Survival was defined as the point at which animals could not right themselves within 30s after being placed on their side.