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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Mitochondrial permeability transition pore induces mitochondria injury in Huntington disease

Figure 3

Effects of FK-506 on mitochondria impairment induced by thapsigargin in mutant huntingtin cells. A, wild type and mutant cells were incubated with 500 nM FK-506, an inhibitor of calcineurin activity, for 2 h prior to mitochondrial potential determinations using MitoRed. Striatal cells loaded with MitoRed were exposed to 1 μM thapsigargin (Th) for 30 min, and mitochondrial potential was evaluated using confocal microscopy. Treatment with thapsigargin reduced mitochondrial potential levels in mutant striatal cells pre-treated with FK-506, indicating that calcineurin activity was not playing a role in mitochondrial impairment induced by calcium stress in mutant huntingtin cells. B, quantitation of MitoRed intensities, which represents mitochondrial potential levels, as relative units at 30 min (ΔF/F0) in striatal cells treated with conditions indicated. Data are expressed as the mean ± S.E.M. of 4 independent experiments. *, p < 0.05 compared with wild type cells treated with thapsigargin; ** p < 0.05 compared with wild type cells treated with thapsigargin.

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