Figure 1
From: Features of wild-type human SOD1 limit interactions with misfolded aggregates of mouse G86R Sod1
WT-hSOD1 does not co-sediment with aggregates formed by G86R-mSOD1. A) Immunoblot of detergent-insoluble (P2) and soluble (S1) fractions of HEK293FT cells transfected for 48 hours with vectors for WT-hSOD1 (hWT), A4V-hSOD1 (A4V), G86R-mSod1 (G86R), or co-transfected with 2 vectors (hWT + G86R and GFP + G86R). The GFP vector served as a co-transfection control. Note that G86R-mSod1 migrates faster than the hSOD1 constructs as noted on right side of the Figure. B) Quantification of the relative aggregation propensity of each transfected protein. Paired student t-tests were performed to establish significant differences with hWT: *p ≤ 0.05, #p ≤ 0.005. Bars represent mean ± SEM. Note that the aggregation propensity of WT-hSOD1 in cells co-transfected with G86R-mSod1 is not significantly greater than when expressed alone.