Figure 3
From: Features of wild-type human SOD1 limit interactions with misfolded aggregates of mouse G86R Sod1
G86R-mSod1 fused with either RFP or YFP forms saponin-resistant inclusions. (A) G86R-mSod1:RFP and (B) G86R-mSod1:YFP proteins were expressed in CHO cells for 24 h. Cells were fixed directly or treated with 0.1% Saponin prior fixation. Cells were immunostained with DAPI to allow visualization of cells nuclei. Note the camera exposure times used to capture RFP and YFP images were recorded as indicated in the figure. Digitally enlarged insets are shown to the right.