Figure 6
From: Features of wild-type human SOD1 limit interactions with misfolded aggregates of mouse G86R Sod1
Mutations at residues F50E/G51E of SOD1 produces a variant of SOD1 that is unable to dimerize with itself or heterodimerize with native WT-hSOD1 subunits. CHO cells were transiently transfected with indicated split luciferase constructs (illustration) and as explained in Methods. A coelenterazine assay was used in order to demonstrate the luciferase activity of the split luciferase constructs. Each assay was repeated at least 3 times and luminescence values were quantified. For each independent experiment, the values were normalized to the value obtained when fusions of each segment of Gluc fused to WT-hSOD1 were co-transfected.