Features of wild-type human SOD1 limit interactions with misfolded aggregates of mouse G86R Sod1

Table 1 Comparison of assays for interactions between WT and misfolded mutant SOD1

Construct	Detergent insoluble	Saponin-resistant RFP or YFP tagged inclusions
WT-hSOD1	No	WT-hSOD1:YFP	No¹
WT-hSOD1	No	WT-hSOD1:RFP	Yes¹
WT-hSOD1mon	Yes	WT-hSOD1mon:YFP	No²
WT-hSOD1mon	Yes	WT-hSOD1mon:RFP	No²
WT-mSod1	No¹	WT-mSod1:YFP	No
WT-mSod1	No¹	WT-mSod1:RFP	Yes³
WT-mSod1mon	Yes	WT-mSod1mon:YFP	Yes
WT-mSod1mon	Yes	WT-mSod1mon:RFP	Yes
G86R-mSod1	Yes	G86R-mSod1:YFP	Yes
G86R-mSod1	Yes	G86R-mSod1:RFP	Yes
WT-mSod1	?	WT-mSod1:YFP	Yes
+		+
G86R-mSod1	Yes 48 hr	G86R-mSod1:RFP	Yes
WT-mSod1mon	Yes 48 hr	WT-mSod1mon:YFP	Yes
+		+
G86R-mSod1	Yes 48 hr	G86R-mSod1:RFP	Yes
WT-hSOD1	No 48 hr	WT-hSOD1:YFP	No
+		+
G86R-mSod1	Yes 48 hr	G86R-mSod1:RFP	Yes
WT-hSOD1mon	Yes 48 hr	WT-hSOD1mon:YFP	No
+		+
G86R-mSod1	Reduced 48 hr	G86R mSod1:RFP	Yes
G85R-hSOD1	Yes	G85R-hSOD1:YFP	Yes²
G85R-hSOD1	Yes	G85R-hSOD1:RFP	Yes²
WT-hSOD1	Yes 48 hr¹	WT-hSOD1:YFP	No²
+		+
G85R-hSOD1	Yes 48 hr¹	G85R-hSOD1:RFP	Yes²
WT-hSOD1mon	Yes 48 hr	WT-hSOD1mon:YFP	No²
+		+
G85R-hSOD1	Yes 48 hr	G85R-hSOD1:RFP	Yes²
WT-mSod1	?	WT-mSod1:YFP	No⁴
+		+
G85R-hSOD1	Yes 48 hr	G85R-hSOD1:RFP	Yes⁴
WT-mSod1mon	?	WT-mSod1mon:YFP	Yes⁵
+		+
G85R-hSOD1	Yes 48 hr	G85R-hSOD1:RFP	Yes⁵

¹data from Prudencio et al. [19]; ²data from Qualls et al. [23]; ³Additional file 1: Figure S1; ⁴Additional file 1: Figure S4; ⁵Additional file 1: Figure S6.

ISSN: 1750-1326