Figure 4

Microarray analysis of dorsal striatal samples from female D9ΔTrkB mice reveals widespread alterations in gene expression (see Table 2). (a) RT-qPCR assay of Ryr1, Slit2, Egr1, Ntrk3, Vgf, Per2, and Lynx2 mRNAs validated the results of the microarray analysis, whereas assay of Adra2 mRNA did not show a difference between genotypes (N = 4/genotype; * P < 0.05,**P < 0.01,***P < 0.001). (b) RT-qPCR assay of Ryr1, Per2, Ptpn5 and Vgf mRNAs in striatal samples from male mice (N = 4/genotype) showed reductions in transcripts similar to those observed in samples from females (**P < 0.01,***P < 0.001). (c) Venn diagram illustrating that of the top 325 genes with mRNA levels altered at the P < 0.005 level in D9ΔTrkB mice, only 144 were altered in BDNF-null mice (from [18]) at the least stringent level of significance, P < 0.05.