Figure 3
Respiration rates of PD Orig and PD CLB cybrid clones. (A, C, E) OCR was measured using the Seahorse XF24 analyzer for all three PD cybrid pairs and controls (not shown). Oligomycin, FCCP, rotenone, and antimycin A were added at the same time point for each experiment. in sequential to measure specific components of the mtETC (see Methods). (A, B) PD61CLB had significantly higher OCR but not residual (non-mitochondrial) respiration than PD61Orig (n=8). (C, D) PD63CLB had significantly lower OCR than PD63Orig (n=10). (E, F) OCR in PD67CLB did not differ significantly from PD67Orig (n=10). Two-way ANOVA with Bonferroni multiple comparisons; *, p<0.05; **, p<0.01; #, p<0.001.