Summary of manganese (Mn2+) enhanced MRI (MEMRI) preprocessing and analysis procedure. A) Steps in image processing and generation of Z score maps. B) Intensity histograms for raw data (top panel) and data presented as normalized z scores (bottom panel). A comparison is made between control nonTg mice (n = 5; dark line) and rTg4510 mice (n = 5; grey lines). Data presented as mean voxel counts (± standard error). Voxel-wise Z score calculation equation used is shown. Inset in bottom graph highlights upper portion of the histogram accounting for voxels with increased signal intensity (Z ≥ 1). C) Effect of Mn2+ dose on mouse brain signal intensity in control scans normalized using the Z score procedure. Data presented as mean number of voxels at Z scores equal or above 1 (n=6 for 20 mg/kg MnCl2 administration, n=5 for 70 mg/kg MnCl2 administration, respectively). Coronal brain maps to the right of the graph are both at a set signal intensity threshold between Z =−2 and Z = +3. *p = 0.001 unpaired two-tailed t-test.