Manganese enhanced intensity and thickness of hippocampus. A) Representative coronal sections of non Tg and rtg4510 mouse brains. The coronal slices were at the level Bregma −2 mm. Sub-regions of hippocampus were drawn as CA1 (green) and DG (purple). Arrows showed the analyzed position of hippocampus thickness. The position was set at 1.5 mm from center. Scale bar=2 mm. B) Relative intensity of CA1 region was normalized by averaged intensity of whole brain. C) Relative intensity of DG region was normalized by averaged intensity of whole brain. D) Ratio of intensity between CA1 and DG. E) Comparison of hippocampus thickness between non Tg (n=5) and rTg4510 (n=5) mice. **p<0.01, ***p<0.001.