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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase

Figure 2

Dynamic transport of BACE1-YFP in hippocampal mossy fibers of Tg BACE1-YFP mice. (A) Tg BACE1-YFP brain stained with anti-neurofilament NFT160 mAb. Note the prominent localization of BACE1-YFP in axons of hippocampal mossy fibers (MF). Nuclei of neuronal cell bodies were labeled using DAPI staining. (B) Representative 2-photon confocal image of YFP fluorescence in Tg BACE1-YFP hippocampal brain slice acquired near the region used for time-lapse imaging depicted in (B). MF, Mossy fibers. Note BACE1-YFP fluorescence in puncta of dendrites and in the soma of a few CA3 neurons. (C) Time-lapse images of hippocampal slices from Tg BACE1-YFP were acquired at 30°C on a multiphoton confocal microscope at the rate of 0.8 frames/sec for 4 min. Montage of representative images from Additional file 1, and corresponding kymographs are shown. Arrowheads indicate the movement of BACE1-positive vesicular/tubular carriers away from the DG (anterograde) or toward the DG (retrograde). (D) Maximum velocities of BACE1-YFP carriers (n = 136 anterograde; n = 113 retrograde; 8 brain slices from 3 BACE1-YFP bigenic mice imaged) were quantified from kymographs and plotted as a histogram.

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