mRNA expression patterns of selected optic nerve genes following ONC insult. Pooled microarray mRNA expression changes were validated in individual samples by qRT-PCR. Relative fold change in each sample was determined based on a 2 fold exponential using mRNA expression values normalized to Gapdh and the contralateral control eye. Fold values of each gene presented as mean ± SEM. (A) Neuritin 1 (Nrn1), (B) Synaptotagmin 1 (Syt1) and (C) Synaptoporin (Synpr). Statistical significance for each time-point determined by one-way ANOVA -Tukey post hoc test, * p < 0.05, ** p < 0.01, *** p < 0.001, n = 5.