Expression of Syt1, Synpr and Nrn1 in the optic nerve following ONC. Differential protein expression of Syt1, Synpr and Nrn1 observed within cytoplasmic region of ON cells following trauma. Optic nerve sections from naïve, 14, 21 and 28 dpc (days post crush) are indicated on the top of the panel. (A) Illustrative images captured at each time point for each protein. Fluorescence micrographs of optic nerve tissue sections were captured at 600x magnification and immunolabeled with: (B) Syt1 (green), (C) Syt1 and DAPI, (D) Synpr (green), (E) Synpr and DAPI, (F) Nrn1 (green) and (G) Nrn1 and DAPI. Blue staining indicates DAPI labeled nuclei. (Scale bar = 100 μm (100x), 25 μm (600x), n = 3). Photomicrographs were captured at 600x original magnification for the selected ON genes.