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Table 4 Temporal classification of down-regulated ON gene cluster changes following ONC

From: Optic nerve crush induces spatial and temporal gene expression patterns in retina and optic nerve of BALB/cJ mice

Gene ontology Clusters Time point P value
Molecular function Calmodulin binding 3 dpc 6.50E-04
  Voltage gated ion channel activity 3 dpc 6.70E-04
  Ion binding 3 dpc 3.90E-04
  Enzyme binding 3 dpc 5.80E-03
  GABA receptor activity 3 dpc 6.70E-03
  Ligand gated ion channel activity 3 dpc 1.80E-02
  Calmodulin binding 7 dpc 1.30E-02
  Calcium dependent phospholipid binding 7 dpc 1.40E-04
  Nuclease activity 21 dpc 2.50E-02
  Microtubule binding 21 dpc 2.50E-02
  Motor activity 21 dpc 1.80E-02
  Cytokine activity 28 dpc 2.90E-02
Biological process Potassium ion transport 3 dpc 4.80E-05
  Cation transport 3 dpc 5.30E-04
  Neurotransmitter transport 3 dpc 3.80E-03
  Synaptic transmission 3 dpc 1.40E-02
  Calcium ion transport 3 dpc 1.10E-02
  Neurofilament cytoskeleton organization 3 dpc 1.20E-03
  Intermediate filament cytoskeleton organization 3 dpc 2.00E-03
  Neurotransmitter transport 3 dpc 3.80E-03
  MAPKKK cascade 3 dpc 2.40E-02
  Microtubule based process 7 dpc 1.10E-02
  Visual perception 14 dpc 4.40E-05
  Cognition 14 dpc 6.50E-03
  Neurological process 14 dpc 1.40E-02
  Microtubule based process 21 dpc 1.30E-05
  Lipid biosynthetic process 21 dpc 2.20E-02
  Visual perception 28 dpc 3.20E-10
  Sensory perception 28 dpc 2.90E-02
  Eye development 28 dpc 4.80E-05
  Immune response 28 dpc 4.70E-02
Cellular component Synapse 3 dpc 4.30E-06
  Cell junction 3 dpc 6.30E-04
  Post synaptic membrane 3 dpc 1.10E-03
  Presynaptic membrane 3 dpc 5.20E-05
  Synapse part 7 dpc 2.90E-04
  Cell junction 7 dpc 3.90E-03
  Cell projection 7 dpc 3.10E-03
  Clathrin coated vesicle 7 dpc 1.00E-02
  Cytoskeleton 21 dpc 2.30E-02
  Anchored to membrane 28 dpc 4.30E-02
  1. Gene expression fold-change values were grouped individually from naïve eyes and ONC eyes out to 28 days post crush (dpc). Genes were highlighted based on fold values for down-regulated (≤ −1.5) optic nerve datasets. The selected genes were analyzed by gene ontology (GO) based cluster identification at each time point using DAVID. Significance was determined using the Benjamini multiple test correction, GO enrichment score χ2 test and Fishers Exact test (p < 0.05).