Figure 1

6-OHDA rapidly decreases mitochondrial movement in DA axons. A) Diagram of microdevice B) Axonal movement of mitochondria in control and 6-OHDA treated axons. DA-GFP cultures (Top panels) grown in microdevices and transduced with MitoDsRed2 (Middle panels) were imaged 30 minutes after treatment with 6-OHDA. Resulting kymographs are shown below. For additional clarity tracks of moving particles are depicted in the bottom panels: blue lines denote anterograde movement and red lines indicate retrograde trafficking. Scale bar indicates 10 μm. Quantification of C) moving mitochondria (n = 4–5 devices per group with 4–5 axons analyzed per device) and D) mitochondrial speeds. The latter were calculated as described [10] (n = 60–80 mitochondria per group). In C and D, data are represented as mean ± SEM, * + indicates p < 0.05 versus control and 6-OHDA in somal compartment.