Figure 3
From: The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons
6-OHDA rapidly depolarizes mitochondria in both DA and non-DA axons. A) To ensure rapid, even labeling of mitochondria with TMRE (25 nM), axons were assessed after they had exited the microdevice channels. Scale bar indicates 10 μm. B) 6-OHDA significantly decreased mitochondrial membrane potential (ΔΨm) in DA and non-DA axons. Data indicate mean ± SEM from four independent experiments (n = 18–30 axons per group). ** indicates p < 0.001 versus control. C) Quantification of cross-sectional area of DA mitochondria before and after treatment with 6-OHDA. Data indicate mean ± SEM.