Accumulation of CT α-syn and caspase-3 activation in α-syn/APP tg mice. A. Confocal images and B image analysis of increased caspase-3 activation in the hippocampal CA3 region. While caspase-3 was significantly increased in all tg lines compared to non-tg mice, the most dramatic increase was in the α-syn/APP tg mice. C. Photomicrographs and D image analysis of C-terminal α-syn accumulation of α-syn CT fragments. Accumulation of CT-α-syn was significant in the α-syn tg line and was most significantly increased in the α-syn/APP tg line. E. Representative Western blot and F. immunoblot analysis showing increased levels of α-syn CTF and mGluR5 in APP, SYN, and SYN/APP tg mice. * = p-value < 0.05, and ** = p-value < 0.01 by one-way ANOVA and Dunnett’s post hoc analysis compared to non-tg mice. For each analysis, N = 6 (3–4 months old) mice from each line were utilized.