Creating mGLuR5-mediated vulnerability in the CA1 region via lentivirus delivery in vivo. A. Schematic representation of lentivirus expressing either control vector or mGLuR5 that was injected unilaterally in the CA1 of the hippocampus. B. Representative photomicrographs and C image analysis of sections from mice injected with LV-control and immunoreacted with mGluR5, α-syn and MAP 2. Injecting LV-control had no effect on either non-tg or tg mice and was comparable to the initial characterization of these mice in which levels of mGLuR5 remained the same, α-syn increased in α-syn and α-syn/APP tg mice, and MAP 2-ir was unaffected. D. Representative photomicrographs and E. image analysis revealed that injection of the LV-mGluR5 resulted in a significant increase in mGluR5 in the neuronal cell bodies and dendrites in the CA1 of the hippocampus in the non-tg and tg lines. While α-syn remained unaffected by LV-mGluR5 with α-syn increased in α-syn and α-syn/APP tg mice, MAP 2-ir was significantly reduced in dendrites in the CA1 region. * = p-value < 0.05 and ** = p-value < 0.01 by one-way ANOVA and Dunnett’s post hoc analysis compared to non-tg mice. For each analysis, N = 6 (3–4 months old) mice from each line were utilized.