Simplified CLARITY clearing procedure. (A) Hydrogel-embedded coronal slices before (bottom) and after (top) passive clearing procedure. (B) Coronal slices on microscope cover glass prepared for imaging. (C) Confocal images of striatal region co-stained with DARPP32 (green) and PSD-95 (red) antibodies. (D) Confocal images of hippocampus region stained with PSD-95 antibodies (red). Scale bar is 150 microns on panels C-D.