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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Stem cell derived basal forebrain cholinergic neurons from Alzheimer’s disease patients are more susceptible to cell death

Figure 2

Generation of basal forebrain cholinergic neurons from iPSCs. (A) Over 90% of cells derived from SHH/FGF8 treated neural progenitors (Control 1 line shown) are Map2 positive neurons. (B) ChAT positive cholinergic neurons are generated after nucleofection of SHH/FGF8 treated neural progenitors with Lhx8/Gbx1 transcription factors. (C) Quantification of ChAT positive neurons among total Map2 positive neurons in SHH/FGF8 treated and Lhx8/Gbx1 nucleofected culture compared to non-treated, non-nucleofected control group (n = 3, Student’s t-test, ****p < 0.0001, n indicates number of replicative experiments). Further confirmation of basal forebrain cholingeric identity was done by staining with (D) VAChT (E) co-expression of ChAT with Nkx2.1 (F) expression of BFCN specific marker p75-NTR but not spinal cholinergic marker HB9. Scale bars = 16 μm for panel D and 85 μm for other panels. Nucleus is stained with DAPI in blue.

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