Figure 3

Control and AD iPSCs-derived BFCNs express functional voltage-gated sodium and calcium channels. Representative current traces evoked by step depolarization in the absence (A, C) or presence of TTX (B, D) from 2-week old control1 and ApoE3/E4 AD line AG11414 BFCNs, note the elimination of the fast-inactivating inward current in the presence of TTX. Scale bar = 1 nA, 10 ms. (E) Calcium imaging with Fura-2 showed calcium transients evoked by depolarization with 56 mM KCl (black arrow indicates time of addition) of 2-week old BFCNs. ATP was added as a control for metabolically active neurons. (F) 2-week old control and AD BFCNs were briefly exposed to 56 mM KCl and calcium influx was monitored by calcium imaging. Peak amplitude from 25 cells were averaged and normalized to baseline for each group, dashed lines indicate SEM of each data point, n = 3, n indicates number of replicative experiments.