Figure 5

AD-E3/E4 BFCNs are more sensitive to ionomycin or glutamate treatment. (A) 2-week old BFCNs were treated with DMSO, 1 μM or 15 μM ionomycin for 16 hours, live/dead assay was performed and quantified, DMSO control is normalized to 1. (n = 3, mean ± SEM, two-way ANOVA, p = 0.0001, F(2,18) = 20.53; Bonferroni multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n indicates number of replicative experiments from the same line). (B) 2-week old BFCNs were exposed to saline or 50 μM glutamate for 15 minutes and cells were incubated for another 24 hours. Cell viability was quantified by MTT assay. Both sets of data are presented as percentages of living cells. (n = 3, mean ± SEM, two-way ANOVA, p < 0.0001, F(1, 36) = 38.43; Bonferroni multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n indicates number of replicative experiments from the same line).