Skip to main content
Figure 6 | Molecular Neurodegeneration

Figure 6

From: Stem cell derived basal forebrain cholinergic neurons from Alzheimer’s disease patients are more susceptible to cell death

Figure 6

AD-E3/E4 BFCNs have increased cytoplasmic calcium levels upon glutamate stimulation. 2-week old neurons were briefly exposed to 50 μM glutamate and responses were recorded by calcium imaging and measured by ratio of signals at 340 nm/380 nm. Baseline and peak amplitude is measured for each individual cell and cytosolic free calcium levels were calculated as (peak amplitude-baseline)/baseline. (A) Averages of calcium transient of 20–30 cells per experiment were calculated and compared to the average of controls (n = 4, mean ± SEM, ANOVA, p = 0,0005, F(3, 12) = 19.55; Bonferroni multiple comparisons, **p < 0.01, ****p < 0.001), n indicates number of replicative experiments from the same line. Representative images of imaged cells at 340nm (B) and 380nm (C) are shown. Scale bar = 85 μm.

Back to article page