Figure 2

Effects of hyperamylinemia on behavior. The SmartCageTM system was used for automated analysis of spontaneous activity in HIP rats and WT rats. The activity time was measured as infrared photobeam breaks. Each rat was monitored in an ordinary home cage placed within the SmartCageTM platform for 72 hours, including the initial 24 hours for accommodation/ acclimation. After the initial 24 hours of acclimation, recordings were made for the next 48 hours. The recording time 1 started at 1 a.m. Grey area denotes nighttime. A – Total travel distance was similar in HIP rats and WT rats. B – HIP rats showed unchanged ambulatory velocity compared to WT rats. C – Compared to WT rats, HIP rats had decreased exploratory activity demonstrated by the low spontaneous rear up count. D – Vestibulomotor performance in HIP rats and WT rats was assessed by the rotarod performance test. Same animals as in above were subjected to the rotarod performance test at a rod-rotatxing speed increased from 0 rpm to 15 rpm within 1 min. HIP rats showed no ability to improve performance on the rotarod. In contrast, WT rats improved rotarod time with each day of training.