Table 4 PCR primers for rs76719109 and rs592297 AEI assay
From: An intronic PICALM polymorphism, rs588076, is associated with allelic expression of a PICALMisoform
Assay | DNA | Primer Sense | Primer Sequence |
|---|---|---|---|
rs76719109 | cDNA | Sense | 5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGGAGTCAACCAGGTGAAAA |
|  | cDNA | Anti-sense | 5′ CCTCTCTATGGGCAGTCGGTGATTTGGTTGCGTCATTACAGGA |
|  | gDNA | Sense | 5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGGAGTCAACCAGGTGAAAA |
|  | gDNA | Anti-sense | 5′ CCTCTCTATGGGCAGTCGGTGATAGGAGCTTTTTCAACTCACCA |
rs592297 | cDNA | Sense | 5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGAACACAGAAAAACTCCTAAAAA |
|  | cDNA | Anti-sense | 5′ CCTCTCTATGGGCAGTCGGTGATGGCAGCATTTATTACCCCATT |
|  | gDNA | Sense | 5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGAACACAGAAAAACTCCTAAAAA |
|  | gDNA | Anti-sense | 5′ CCTCTCTATGGGCAGTCGGTGATTCTGTGAAAACTTGAGGTTAAAAA |