Overview of gene expression profiles in cortex and hippocampus of 5XFAD mice, at 4 different ages, reveals a shift in expression patterns between M1 and M4. (A) Number of up- (red) and down- (green) regulated genes in cortex and hippocampus of 5XFAD mice compared with wild type animals at M1, M4, M6 and M9. Total number of DEGs for each brain region at each age is reported in brackets. (B) Number of overlapping and non-overlapping DEGs in cortex (blue) and hippocampus (yellow) at M1, M4, M6 and M9. The center of the Venn diagram (green) illustrates the proportion of shared DEGs between both brain regions. (C and D) Number of shared and specific DEGs across all ages in cortex (C) and hippocampus (D). Analysis was based on the total number of DEGs, both up- and down-regulated at each age. Fold change (FC) cut-off used for above analyses was −1.5 > FC > 1.5 when comparing signals from 5XFAD mice with wild type controls.