Figure 1

AAV2-Cre/GFP transduces RGCs in Rosa26-LacZ^fl/fland Rosa26-Tomato^fl/flmice. (A-B) Retinal whole mounts from non-injected (A) and injected (B) eyes of Rosa26-LacZ^fl/fl mice were taken 8 weeks following injection. X-Gal staining indicates global reporter gene expression in injected eyes. (C) Retinal section taken from an injected eye of Rosa26-LacZ^fl/fl illustrates that X-Gal staining is restricted to cells of the ganglion cell layer (GCL) (Scale bar: 20 μm) and is not present in the outer nuclear layer (ONL) and inner nuclear layer (INL). (D) GFP fluorescence carried by AAV2-Cre/GFP is found in the RGC somas and axons (indicated by arrows) in a retinal whole mount (Scale bar: 10 μm). (E) AAV2-Cre/GFP tropism to RGCs in the GCL, and not to the inner plexiform layer (IPL) is shown by nuclear BRN3A (green), TOMATO (red), and DAPI (blue) co-labeling in a retinal section (arrowhead). (Scale bar: 4 μm).