Figure 7
Hdac3 cKO in the RGCs prevented the breakdown of the nuclear envelope and pore integrity following ONC. (A-C) TEM images were taken of nuclear envelopes from Hdac3fl/fl control retinas, Rosa26-Tomatofl/fl crushed retinas, and Hdac3fl/fl crushed retinas. The nuclear lamina is situated at the top of each micrograph. (A) Example of the well-defined inner and outer membranes of the nuclear envelope and normal pore structure of a healthy cell. (B) Example of the nuclear envelope from a cell in a Rosa26-Tomatofl/fl retina 5 days after ONC. The nucleus exhibits formation of electron-dense heterochromatin localized to the inner surface of the nuclear envelope, and the intermembrane space has expanded. (C) The nuclear envelope of a presumptive RGC in an Hdac3fl/fl cKO mouse after ONC. The chromatin appears euchromatic and the nuclear envelop exhibits pore structures, although the apposition of the inner and outer membranes appears wavy. Arrowheads = nuclear pores, small arrows = nuclear envelope double membranes (ne), N = nucleus, and hc = heterochromatin. (Scale bar: 300 nm).