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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Amyloid peptides ABri and ADan show differential neurotoxicity in transgenic Drosophila models of familial British and Danish dementia

Figure 2

Generation of transgenic lines with similar expression levels. A, schematic representation of the fusion peptides used to generate transgenic flies. Amino-acid single-letter code is used. Nec, the signal peptide of necrotic (MASKVSILLLLTVHLLAAQTFAQ); (H)6 indicates the 6 × His-tag. The arrow indicates the site of cleavage in the secretory pathway. The rectangle encloses the sequence shared by Bri2-23, ABri and ADan. B, agarose gels showing the PCR products obtained from genomic DNA of transgenic lines carrying one copy of untagged (arrowheads) or two copies: one untagged and the other with a 6 × His-tagged peptide DNAs (arrows). On the right, molecular markers in base-pairs (bp). C, similar levels of mRNA expression of Bri2-23, ABri and ADan in the eye as determined by QRT-PCR normalized with tubulin mRNA. Bars represent the mean ± SEM of 3 independent experiments. A.U., arbitrary units.

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Molecular Neurodegeneration

ISSN: 1750-1326