Figure 4
Differential toxicity of Aβ42, ABri and ADan in the eye. A, morphological analysis of eye integrity from flies expressing Bri2-23, Aβ42, ABri and ADan. Top panel, ESEM microphotographs indicating the selected area (135 × 135 μm) used to count the number of ommatidia and bristles.The area used for quantification of fused ommatidia was slightly larger (195 × 170 μm) and it is not shown for clarity. Bottom panel, higher magnification of the selected regions as delineated in the top panel. Fused ommatidia are indicated with arrows. Bar scales, 100 μm and 50 μm for top and bottom panels, respectively. B, quantification of fused ommatidia in the selected area for each genotype. C, quantification of bristles in the selected area for each genotype. One- way ANOVA followed by Dunnett’s comparison test analysis was performed. Eight eyes per genotype, randomly taken from 5 independent biological experiments were analyzed. Data are expressed as mean ± SEM. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).