LPS-stimulated mRNA and NOx production in HuNOS2tg/mNos2-/-mice . A. Comparison of the fold changes in NOS2 mRNA levels for HuNOS2 and mNos2 (WT) mice. Brain lysates were prepared from saline injected and LPS injected WT and HuNOS2tg/mNos2-/- mice at 7 and 24 hrs. Average fold changes (±sem) for LPS-treatment were determined using the average saline-injected value as comparator. n = 3 mice/group. ** = p <0.01 using the unpaired student’s t test with significance set at p < .0.05. B. Brain lysate levels of nitrite and nitrate (NOx) were measured in HuNOS2tg/mNos2-/-, mNos2-/- and WT mice at 7, 18 and 24 hrs. Saline- injected mice from each strain served as the 0 hrs time point. Average NOx values (ng/ug protein ± sem) are shown where n = 6-9 mice/strain per time point. Significance with time within strain was determined using one-way ANOVA while statistical significance across genotypes was determined using two-way ANOVA (GraphPad Software, San Diego CA). Significance was set at p ≤0.05. *p <0.05; ***p <0.001 for comparisons across strains; ###p <0.001 for comparisons within strain. C. Analysis of NOx in liver lysates from the same mice as above and under the same conditions. ***p <0.001 for comparisons across strains; ###p <0.001 for comparisons within strain.