Figure 3

Comparison of redox gene expression levels between LPS-simulated HuNOS2^tg/mNos2^-/-mice and WT mice. HuNOS2^tg/mNos2^-/- and WT mice were injected with 10 mg/kg LPS, or with saline as a control, and brains were removed after 7 or 24 hours. RNA was extracted, reverse transcribed, and target gene expression was measured by q-PCR. Fold changes (RQ) were determined for HuNOS2^tg/mNos2^-/- and WT mice using appropriate saline-injected animals as the comparator for each mouse strain. RQ values were linearized by Log2 conversion, and then subjected to conditional formatting whereby red cells denote lower expression and green cells denote higher expression according to the color scale. Significant differences in gene expression for LPS treated as compared to same strain saline-injected controls were determined by student’s t-test (*p ≤0.05, **p <0.01, ***p <0.001). Significant differences in gene expression between WT and HuNOS2^tg/mNos2^-/- mice at specific time points were determined by student’s t-test (Δ p ≤0.05, ΔΔ p <0.01, ΔΔΔ p <0.001). n = 3 mice per group.