Comparison of gene expression for selected genes. Genes that showed changes in expression levels between WT and HuNOS2tg/mNos2-/- mice were also examined for expression levels in mNos2-/- mice. Brain lysates were prepared from saline-injected (white bars) and LPS-injected (black bars) mNos2 knockout mice as described and gene expression measured using qRT-PCR. Average fold changes (±sem) for each gene in saline and LPS-treated conditions were determined using the average WT saline-injected value as comparator. Statistical significance across genotypes and treatment was determined using two-way ANOVA (GraphPad Software, San Diego CA). Significance was set at p ≤0.05. *p <0.05; **p <0.01 for comparisons with treatment; #p <0.05, ##p <0.01 for comparisons across strains. n = 5–6 mice per group.