Figure 5
Changes in immune gene expression levels in LPS-simulated HuNOS2tg/mNos2-/-; APPSwDI+/-/Hu NOS2tg+/+/mNos2-/-; mNos2-/-, and WT mice. Brain lysates were prepared from saline-injected (gray bars) and LPS-injected (black bars) mice from each of the 4 different strains at 24 hrs. Saline-injected mice from each strain served as the corresponding 0 hrs time point. Average fold changes (±sem) for each gene were determined using the average WT saline-injected value as comparator. n = 5–6 mice per group. Panels A-F- cytokine genes; Panels G-I- chemokine genes - J- gene marker of activated microglia- integrin alpha10 (CD11c). Statistical significance was determined using two-way ANOVA with genotype as the between strain factor and treatment as the within strain factor (GraphPad Software, San Diego CA). Significance was set at p ≤0.05. *p <0.05; **p < 0.01; ***p <0.001; #p <0.05; ##p <0.01; ###p <0.001. K- APP mutations that increase Aβ production and amyloid deposition do not increase brain NOx levels. Brain lysates were prepared from saline injected and LPS injected APPSwDI/mNos2-/- and APPSwDI+/-/Hu NOS2tg+/+/mNos2-/- mice (see methods for details on mouse strains). NOx brain lysate levels in LPS-stimulated WT mice serve as the positive control for NO production. Data are presented as the average NOx values ± sem (n = 4–6 mice per group). Significant differences were determined for each tissue type separately using two-way ANOVA with genotype as the between strain factor and treatment as the within strain factor. #p <0.05.