Figure 6
Mouse stain specific differences in brain microhemorrhage. The total number of microhemorrhages per brain section were calculated for HuNOS2tg+/+/mNos2-/-; mNos2-/-, and WT mice under saline treated (control-gray bars) and LPS-treated (black bars) conditions. Panel A; B- typical view of small (<20 microns; A) and large (>20 microns; B) microbleeds observed in brain sections using Prussian blue histochemistry to identify hemisedrin deposits. Panel C; D- Data represent the average number (+ sem) of microbleeds/section (n- 4-7 mice of each strain) for control and LPS-treated mice. Significant differences were determined using two-way ANOVA with genotype as the between strain factor (^p<0.05) and treatment as the within strain factor (#p < 0.05; ##p<0.01).