Figure 4

Aβ 1-42 disrupts cytoskeleton in transgenic fly at 100pM. Aβ1-40 or Aβ1-42 was diluted in 4% BSA and injected into the body cavity of late embryo of GFP-tau (bovine Tau) transgenic fly (AEL-21-22 hr). 4 days later, the central nerve cord was dissected out, and mounted in PBS and imaged directly with a confocal microscope. Stacks of 20–40 0.5 μm confocal sections were generated. The results for each section were assembled as a stack. At 100pM, damage to the structure of cytoskeleton was observed in Aβ1-42 treated flies but not in flies receiving the Aβ1-40 treatment. At 10nM, both Aβ1-40 and Aβ1-42 led to disruption of the cytoskeleton.