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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Tau pathogenesis is promoted by Aβ1-42 but not Aβ1-40

Figure 5

Higher level of total tau, phospho-tau and cleaved tau in P301S mice receiving an Aβ 1-42 injection into the entorhinal cortex as compared with Aβ 1-40 injection. 8 month old P301S mice were injected with Aβ1-40 into the left entorhinal cortex and Aβ1-42 into the right entorhinal cortex. 18 days after injection, the brain extracts were analyzed by western blot. A) Representative images of western blots of soluble tau (S1 fraction) detected with anti-tau antibodies and normalized to actin. B) Quantification of western blots in (A). C) Representative images of western blots of insoluble tau (P1 fraction) detected with anti-tau antibodies. Both S1 brain fraction and P1 brain fraction show a significant increase in HT7, PHF-1 and pS262 signal in entorhinal cortex injected with Aβ1-42 compared with Aβ1-40 injected entorhinal cortex. Also more cleaved tau (Tau421) was detected in the S1 fraction from the Aβ1-42 injected brain. D) Quantification of western blots in (C). Histographs show mean +/− standard error, n =3. *p <0.05; **p <0.01; ***p <0.005.

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