Figure 4

Quantitative analysis of silver stain images. Silver stained images were quantified by calculating the % of region of interest (ROI) in the white matter tract area that was stained with silver. Bars indicate mean ± SD percent of ROI showing positive signal in sham and rTBI-induced animals in (A) olfactory nerve layer, (B) corpus callosum and (C) optic tract. Data were analyzed using two-way ANOVA followed by a Tukey post-hoc test. Cohort size: olfactory nerve layer: Sham (2d: N = 4, 7d: N = 5, 14d: N = 6); rTBI (2d: N = 8, 7d and 14 d: N = 5); corpus callosum: Sham (2d and 7d: N = 5, 14d: N = 6); rTBI (2d: N = 16, 7d: N = 5, and 14 d: N = 6); optic tract: Sham (2d: N = 5, and 7d: N = 4, 14d: N = 6); rTBI (2d: N = 17, 7d and 14d: N = 5). For all graphs, * indicates a significant rTBI effect within a particular time point and # indicates a significant time effect within rTBI group. **: p < 0.01, ***: p < 0.001, ****: p < 0.0001. ###: p < 0.001, ####: p < 0.0001.