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Figure 8 | Molecular Neurodegeneration

Figure 8

From: Merging pathology with biomechanics using CHIMERA (Closed-Head Impact Model of Engineered Rotational Acceleration): a novel, surgery-free model of traumatic brain injury

Figure 8

CHIMERA rTBI increases endogenous tau phosphorylation. Tau phosphorylation was analyzed using the Simple Western system (ProteinSimple). The graphs in the left column (A-C) depict fold change in endogenous phosphorylated tau levels in rTBI half-brain homogenates compared to the sham brains using antibodies CP13 (pSer202 and pThr205, Panel A), RZ3 (pThr231, Panel B) and PHF1 (pSer396 and pSer404, Panel C), respectively. Graphs in the middle column (D-F) depict quantitation of phosphorylated tau as a proportion of total tau (DA9). Representative digital immunoblots of phosphorylated and corresponding total tau are depicted in the right column (G-I). Arrows on the left of the blots indicate molecular weight marker at 66 kDa. Data are presented as the mean ± SD fold change in rTBI compared to the respective shams at each time point. For all graphs, *, ** and *** indicate p < 0.01 for the comparison of rTBI vs respective sham values, using multiple t-tests with Bonferroni correction for multiple comparison (p = 0.05/5 = 0.01).

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