Figure 2

Analysis of signalling pathways from proteomic experiments. Venn diagrams of deregulated proteins from cortex [C] (A) and hippocampus [H] (B) exposed to 0.02 Gy, 0.1 Gy, 0.5 Gy and 1.0 Gy from global proteomics approach are shown. H: n = 4; C: n = 5. The number above each dose shows the total number of deregulated proteins at this dose. The unique and overlapping significantly deregulated proteins in hippocampus and cortex at doses of 0.5 Gy and 1.0 Gy are shown in panel C. Each column shows the proteins significantly up-regulated (bold) or down-regulated (italic) with the fold-changes in brackets. Proteins indicated with a and/or b belong to the protein class of “small GTPase/associated G-protein” and/or “cytoskeleton / cytoskeleton-binding protein”, respectively, as categorised using the PANTHER software tool and UniProt database. Hippocampal and cortical data result from four and five biological replicates. Associated signalling pathways of all dose-dependent significantly deregulated proteins using the Ingenuity Pathway Analysis (IPA) software are shown in panel D. Higher colour intensity represents higher significance (p-value) whereas all coloured boxes have a p-value of ≤ 0.05; white boxes have p-value of > 0.05 and are not significant. Hippocampal and cortical data result from four and five biological replicates, respectively. H: Hippocampus, C: Cortex. Panel E shows the overlapping proteins within the in panel D depicted signalling pathways.