Figure 5

Quantification of the expression of genes and proteins involved in synaptic plasticity. Genes significantly changed in expression from hippocampus and cortex at doses of 0.5 Gy and 1.0 Gy using RT2 Profiler PCR Arrays are shown in A. The table shows the genes which are significantly up-regulated or down-regulated with the fold-changes in brackets; *p < 0.05; **p < 0.01; ***p < 0.001 (unpaired Student’s t-test, n = 3) after normalisation to the median of 84 target genes. Immunoblots and quantification of Arc, c-Fos, total CREB and phosphorylated CREB in hippocampus and cortex of sham-irradiated and 1.0 Gy irradiated mice 7 months post irradiation (B and C). The columns represent the fold-changes with standard errors of the mean (SEM) from three biological replicates. The visualisation of protein bands shows the representative change using three biological replicates. *p < 0.05; **p < 0.01; ***p < 0.001 (unpaired Student’s t-test). Normalisation was performed against endogenous GAPDH; H: Hippocampus, C: Cortex.