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Figure 6 | Molecular Neurodegeneration

Figure 6

From: The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation

Figure 6

Adult neurogenesis and evaluation of apoptosis, DNA strands and derivations in animal and brain weights. Schematic representation of proneural markers for staging adult neurogenesis in the hippocampus by immunohistochemistry is shown (A). Representative image of stem-like cells (type 1 radial glial) labelled by GFAP (B), of proliferating progenitor cells (type 2a) labelled with PCNA (D) and Ki67 (F), of non-radial stem cells (type 2b) labeled by Sox2 (H), of newborn and mature neurons labeled by Dcx (J) and NeuN (L) and corresponding relative quantifications (C, E, G, I, K, M) are shown. NeuN+ cells were counted in 4000 μm2 areas (in green). *p < 0.05; **p < 0.01; ***p < 0.001 (unpaired Student’s t-test). Data are reported as mean ± SEM (n = 3 for Ki67, n ≥ 3 for NeuN, others n = 6); DG, dentate gyrus; CR, crest area; SB, suprapyramidal blade; IB, infrapyramidal blade. Visualisation of activated Caspase-3 (N) and γH2AX (O) in hippocampus and DG and fold-changes of brain weight, body weight and their ratios (P) are shown. The positive control of γH2AX consisted of hippocampus irradiated with 3.0 Gy and stained after 30 minutes. Data of weights are reported as fold-changes ± SEM from at least 5 biological replicates (Student’s t-test, unpaired).

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