Figure 2

Membrane cholesterol loading causes neuronal EEA1-positive early endosomes enlargement but does not impact on their number, as assessed by confocal microscopy. (A) and (B) Representative confocal images of cortical neurons stained with an anti-EEA1 antibody in control conditions (A) or after cholesterol loading (B). (C) Mean number of endosomes per soma (ns stands for p > 0.05 in unpaired Student’s t-test; n = 46 control neurons and 45 cholesterol-treated neurons observed in 3 independent experiments). (D) Mean endosomal size (*** stands for p < 0.001, Mann–Whitney test, 4171 control endosomes and 4248 cholesterol-treated endosomes, collected in 3 independent experiments). (E) Endosome size distribution of small-, medium- and large-sized endosomes, based on κ-means clustering and normalized to control conditions.