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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Increasing membrane cholesterol of neurons in culture recapitulates Alzheimer’s disease early phenotypes

Figure 4

Membrane cholesterol loading inhibits APP vesicular trafficking in neuronal processes. (A-F) Representative APP-mCherry expression in neuritic processes and kymographs obtained from live-imaging experiments in control condition (A and D), after neurobasal medium alone addition (B and E) or after MβCD-cholesterol dissolved in neurobasal medium treatment (C and F). (G) Proportion of stationary APP vesicles (ns stands for p > 0.05 in unpaired Student’s t test, n = 52 control and 24 neurobasal-treated neurons and ** for p < 0.01 in unpaired t test with Welch’s correction, n = 52 control and 30 cholesterol-treated neurons). (H) Mean velocity of the remaining moving vesicles. For retrograde transport, ns stands for p > 0.05 in one-way ANOVA with Tukey’s post-test (n = 595 measures for control, 218 measures for neurobasal-treated and 268 measures for cholesterol-treated neurons). For anterograde transport, ns stands for p > 0.05 and *** for p < 0.001 in one-way ANOVA with Tukey’s post-test (n = 726 measures for control, 365 measures for neurobasal-treated and 241 measures for cholesterol-treated neurons).

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